mouse primary brain microvascular ecs #c57-6023  (Cell Biologics Inc)

Journal: Vascular Cell

Article Title: The role of microRNAs in neural stem cell-supported endothelial morphogenesis

doi: 10.1186/2045-824X-3-25

Figure Lengend Snippet: Changes in miR-155, miR-100, and miR-let-7i expression affect in vitro endothelial morphogenesis . 24 hours after transfection with specific synthetic inhibitors (A) or mimics (B), ECs were subjected to in vitro EC morphogenesis assay. DIC images of unlabeled cells were acquired using a Nikon TE2000 microscope and SlideBook software (DIC micrographs). GAPDH-labeled low-magnification images of the tubular structures (red, inserts) were acquired using a Zeiss LSM confocal microscope. Bars 20 μm. Graphs: Data are expressed as the average tube length, or number of branch points per visual area ± S.E.M., n = 10 wells per inhibitor/10 images per well. Student's t-test: * P < 0.05; **P < 0.01; *** P < 0.001.

Article Snippet: Mouse brain endothelial primary cells (ECs) were purchased from Celprogen (San Diego, CA).

Techniques: Expressing, In Vitro, Transfection, Microscopy, Software, Labeling

Journal: Vascular Cell

Article Title: The role of microRNAs in neural stem cell-supported endothelial morphogenesis

doi: 10.1186/2045-824X-3-25

Figure Lengend Snippet: Hypothesis on the possible mechanisms of the pro-angiogenic effect produced by NSPCs . A: general mechanism of the NSPC-supported EC morphogenesis. NSPC-released pro-angiogenic factors induce changes in miRNA and gene expression in ECs, leading to activation of cell signaling pathways regulating EC morphogenesis and blood vessel formation. Panel B: a possible link between NSPC signaling and alterations in EC miRNA expression, leading to endothelial morphogenesis. NSPC-released pro-angiogenic factors such as VEGF, TGF-β and TNF, etc., bind to the corresponding receptors on EC surfaces, and trigger the activation of transcriptional or post-transcriptional supressors of miRNA 155, 100, and let-7i expression. Resulting downregulation (blue arrow) of miR-155, miR-100, and miR-let-7i leads to the increased (red arrow) expression of mTOR, SMAD2, and SMAD3, and thus activation of the mTOR and TGF-β signaling cascade and transcription of genes responsible for endothelial morphogenesis.

Article Snippet: Mouse brain endothelial primary cells (ECs) were purchased from Celprogen (San Diego, CA).

Techniques: Produced, Expressing, Activation Assay

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet: The distance between the endothelial cells in the insert and the cardiomyocytes at the bottom of the well can be varied by using different inserts. All three types of inserts used here have the same pore size of 0.4 μm. The only difference among them is the insert-to-base height, which allows the distances between the two co-cultured cell layers to be 0.5 (A), 1.0 (B) and 2.0 mm (C), respectively.

Article Snippet: 29 , Mouse Primary Coronary Artery Endothelial Cells (ECs) , Cell Biologics Inc , C57–6093 , Isolated from coronary artery of C57BL/6 mice.

Techniques: Pore Size, Cell Culture

Journal: Journal of visualized experiments : JoVE

Article Title: Development of a Cell Co-Culture Model to Mimic Cardiac Ischemia/Reperfusion In Vitro

doi: 10.3791/62913

Figure Lengend Snippet:

Article Snippet: 29 , Mouse Primary Coronary Artery Endothelial Cells (ECs) , Cell Biologics Inc , C57–6093 , Isolated from coronary artery of C57BL/6 mice.

Techniques: Isolation, Cell Counting, Cell Culture, Sterility, Co-Culture Assay, Passaging, CyQUANT Assay, Microscopy, Saline

Journal: Frontiers in Pharmacology

Article Title: Engineered multi-functional, pro-angiogenic collagen-based scaffolds loaded with endothelial cells promote large deep burn wound healing

doi: 10.3389/fphar.2023.1125209

Figure Lengend Snippet: Characterization of mouse bone marrow derived endothelial cells (ECs). (A) Flow cytometry of CD31, CD34, CD45, CD144 and CD90 expression and GFP transduction on ECs. (B, C) Immunofluorescence staining results of expression of CD31 (B) and VE-Cadherin (C) . (D) Acetylated low-density lipoprotein uptake by ECs. (E) Representative phase contrast image of in vitro tube formation of ECs. Scale bar = 200 μm.

Article Snippet: C57BL/6 mouse primary bone marrow-derived endothelial cells (ECs) were purchased from Cell Biologics, Inc. (C57-6221).

Techniques: Derivative Assay, Flow Cytometry, Expressing, Transduction, Immunofluorescence, Staining, In Vitro